Additional file 3.
qRT-PCR validation of microarray results. qRT-PCR expression comparison of 9 selected genes from the microarray data; (1) estExt_Genewise1_v1.C_1240151,(2) estExt_Genewise1_v1.C_1700234,(3) gw1.XVI.2910.1,(4) eugene3.00121203,(5) gw18.104.22.168,(6) gw1.IX.897.1,(7) fgenesh4_pg.C_LG_III000388,(8) fgenesh1_pg.C_scaffold_385000002 and (9) estExt_fgenesh4_pm.C_LG_III0736. Genes 1 to 3 are from the gene cluster with high IN9 expression (Figure 4); genes 4 to 6 are from the IN2 high expression cluster, and genes 7 to 9 are from the IN5 high expression cluster. The fold-changes for genes 1 to 3 are between those found for IN9 and IN2, for genes 4 to 6 are between those found for IN2 and IN9 and for genes 7 to 9 are between those found for IN5 and IN2. The error bars on the qRT-PCR data column represent the standard deviation between the averages of the three biological replicates.
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Dharmawardhana et al. BMC Genomics 2010 11:150 doi:10.1186/1471-2164-11-150