Additional file 4.
Primers used in this study. The orientation (forward or reverse) of all primers is indicated by 'f' or 'r' in the end of each primer name. Primers whose name is 'Gene--f or r' were used for PCR detection of each putative gene fragment from BAC clones. Primers for quantitative PCR of 4 putative genes (gene 01, 09, 16 and 27) in the BAC clone Mj024A04 and TGase for internal control are indicated by 'qt' at the beginning of each primer name. Primers for three microsatellite markers are indicated by 'MS' at the beginning of each primer name. Three microsatellites repeat regions (02, 33 and 64) were determined by RepeatMasker program . Forward primers (f) were labeled by 6-FAM and reverse primers (r) were designed with tailed primer (Applied Biosystems).
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Koyama et al. BMC Genomics 2010 11:141 doi:10.1186/1471-2164-11-141