Additional file 1.

Coefficient of variation amongst replicate UHRR samples. Two box and whiskers plots of the coefficient of variation (CV) of the replicate UHRR samples. The first plot (A) shows the experiment-wide CV of the UHRR samples. The left-most of the four main sections shows the CV of the raw (detection filtered) data, to the right of this is the CV after four popular normalisation algorithms; quantile, loess, cubic-spline (qspline), and median. The final two segments show the CV after batch-correcting each normalised dataset using either mean-centring or ComBat. In the second plot (B), from the left, the first four segments contain five box-plots illustrating the CV within each of the five runs; the four segments containing raw (white), quantile-normalised (dark-blue), mean-centred (lighter-blue), and ComBat-corrected (pale-blue) data respectively. All data were detection-filtered prior to analysis. The right-most segment shows the experiment-wide CV of the UHRR (coloured as the previous segments) calculated with no consideration of the individual runs.

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Kitchen et al. BMC Genomics 2010 11:134   doi:10.1186/1471-2164-11-134