Table 3

Bacterial strains and plasmids used in this study.

Strain or plasmid

Relevant characteristics

Source or reference


C. glutamicum ATCC 13032

wild-type strain

ATCC

C. glutamicum JS2502

ATCC 13032 with defined deletion in zur

This study

E. coli DH5αMCR

E. coli strain used for standard cloning procedures

[73]

E. coli TOP10

E. coli strain used for cloning of RACE-PCR products

Invitrogen

pCR2.1-TOPO

lacZα, Apr; E. coli cloning vector

Invitrogen

pK18mobsacB

sacB, Kmr; E. coli vector for allelic exchange

[74]

pK18mobsacBzur

sacB, Kmr; pK18mobsacB carrying a modified zur gene with internal deletion

This study

pASK-IBA5+

PTet, strep-tag, Apr; E. coli expression vector

IBA Tagnologies

pASK-IBA5+_cg2502

pASK-IBA5+ carrying the C. glutamicum zur gene

This study

pEPR1

gfpuvPL, Kmr; promoter-probe vector

[28]

pEPR1_prom_cg2500

gfpuvPL, Kmr; pEPR1 carrying the znr upstream region

This study

pEPR1_prom_cg2502

gfpuvPL, Kmr; pEPR1 carrying the zur upstream region

This study

pEPR1_prom_cg0042

gfpuvPL, Kmr; pEPR1 carrying the cg0042 upstream region

This study

pEPR1_prom_cg2911

gfpuvPL, Kmr; pEPR1 carrying the cg2911 upstream region

This study

pEC-XK99E

Ptrc, lacI, Kmr; C. glutamicum expression vector

[75]

pEC-XK99E_zur

Ptrc, lacI, Kmr; pEC-XK99E vector carrying the zur gene for complementation

This study


Schröder et al. BMC Genomics 2010 11:12   doi:10.1186/1471-2164-11-12

Open Data