Table 2

Differentially expressed genes in the zur mutant C. glutamicum JS2502 detected by DNA microarray hybridization and lacking candidate Zur-binding sites.

CDS

Gene

Predicted function

Differential gene expression1

Array

RT-PCR2


cg0045

-

ABC-type transporter, permease subunit

- 1.12

n.s.

cg0215

cspA

cold-shock protein A

1.24

n.s.

cg0793

-

putative secreted protein

1.67

4.01

cg0796

prpD1

citrate dehydratase

1

6.68

cg1109

porB

anion-specific porin precursor

1.12

7.67

cg1332

-

putative secreted protein

1.42

2.57

cg1447

-

putative Co2+/Zn2+/Cd2+ efflux transporter

3.16

25.8

cg1670

-

hypothetical protein

1.07

4.81

cg2181

-

ABC-type transporter, substrate-binding protein

1.1

4.91

cg2261

amtB

secondary ammonium transporter

- 1.01

n.s.

cg2560

aceA

isocitrate lyase

1.56

n.s.

cg2925

ptsS

phosphotransferase system component

- 1.35

n.s.

cg3096

ald

acetaldehyde dehydrogenase

1.69

103

cg3138

-

putative membrane protease subunit

3.14

n.s.

cg3139

-

hypothetical protein

2.03

n.s.

cg3140

tagA1

DNA-3-methyladenine glycolase I

1.88

n.s.

cg3195

-

putative flavin-containing monooxygenase

1.44

3.22


1 Gene expression in C. glutamicum JS2502 was compared with that of the wild-type strain ATCC 13032. Array, m-values obtained by DNA microarray hybridizations (intensity ratio); RT-PCR, values obtained by RT-PCR (relative expression).

2 Abbreviation: n.s., no significant differences detected.

Schröder et al. BMC Genomics 2010 11:12   doi:10.1186/1471-2164-11-12

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