Open Access Highly Accessed Research article

Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus

Rebecca L Skalsky1, Dana L Vanlandingham3, Frank Scholle2, Stephen Higgs3 and Bryan R Cullen1*

Author Affiliations

1 Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA

2 Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA

3 Department of Pathology and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA

For all author emails, please log on.

BMC Genomics 2010, 11:119  doi:10.1186/1471-2164-11-119

Published: 18 February 2010

Additional files

Additional file 1:

Figure S1, miRNA quantification in primer extension experiments shown in Figure 3. Primer extension experiments were quantified using NIH ImageJ. Signal ratios of (A) C7/10-WNV replicon cells: C7/10 cells and (B) WNV-infected Culex: uninfected Culex are graphed for individual miRNAs.

Format: PPT Size: 36KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 2:

Table S1, miRNA reads in Cx. quinquefasciatus and WNV-infected Cx. quinquefasciatus. Table comparing mosquito miRNA counts from high-throughput sequencing of uninfected and WNV-infected Cx. quinquefasciatus. The WNV-infected Cx. quinquefasciatus library was prepared as described in Methods. Differences in miR-989 and miR-92 expression levels are highlighted. nd = not determined

Format: DOC Size: 103KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

Table S2, Oligonucleotides used in this study. Table of oligonucleotides used for primer extension and high-throughput sequencing.

Format: DOC Size: 29KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Raw sequence data C710.fasta. FASTA file containing sequencing reads for C7/10 Ae. albopictus cells

Format: FAST Size: 1.9MB Download file

Open Data

Additional file 5:

Raw sequence data Culex.fasta. FASTA file containing sequencing reads for Cx. quinquefasciatus mosquitoes

Format: ZIP Size: 2.4MB Download file

Open Data

Additional file 6:

miRNA precursor sequences Aedes_precursors.fasta. FASTA file containing miRNA, miRNA*, and precursor sequences for Ae. albopictus

Format: FAST Size: 13KB Download file

Open Data

Additional file 7:

miRNA precursor sequences Culex_precursors.fasta. FASTA file containing miRNA, miRNA*, and precursor sequences for Cx. quinquefasciatus

Format: FAST Size: 13KB Download file

Open Data