Figure 5.

The population of ORFs with 5' rare codon clusters does not show a dependence on the stability of mRNA folding near the 5' terminus. The energetics of mRNA folding for nucleotides 1-40 were calculated for every ORF in E. coli and the population was separated based on the presence (gray circles, left Y-axis) or absence (black squares, right Y-axis) of a rare codon cluster in the first 13 windows, the windows that would overlap with nucleotides 1-40. The ORFs with or without 5' rare codon clusters have a similar median (ΔGfolding: -5.6 kcal/mol and -5.9 kcal/mol, respectively) and the same population distribution as seen by the linear regression when comparing the population with rare codons and those without for each ΔGfolding value (inset).

Clarke and Clark BMC Genomics 2010 11:118   doi:10.1186/1471-2164-11-118
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