Analysis of ornithine decarboxylase 1 (ODC1) and asparagine synthase (ASyn) splice variants in Chlamydomonas using RT-PCR. A) DNAase-treated RNA was used in PCR with ODC1 primers. B) cDNA amplified with ODC1 primers. An asterisk indicates the spliced form for the full-length protein. Numbers on the right indicate amplified product size in Kb. C) cDNA amplified with ASyn primers. Numbers on the right indicate amplified product size in Kb. D) Diagram showing splicing events in six splice variants (left) and predicted proteins (right) for ODC1. Gene is indicated on top and all six splice variants are shown under the gene. Black boxes indicate constitutively spliced exons and red boxes indicate the included regions in different isoforms. Asterisk indicates the position of translation termination codon. Isoforms 1 to 6 correspond to the bottom to top bands in 3B. The number next to each predicted protein indicates the length of the protein. Conserved signature motifs in ODC1 are represented as green boxes in the full-length protein. Red, blue and magenta colors in truncated proteins represent amino acids unique to them. E) Diagram showing splicing events in four splice variants (left) and predicted proteins (right) for the gene ASyn. The representation of the gene and its splice variants is the same as in D. A conserved domain in ASyn is shown in pink. The green region in isoform 4 represents a unique sequence.
Labadorf et al. BMC Genomics 2010 11:114 doi:10.1186/1471-2164-11-114