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Open Access Research article

Analysis of a normalised expressed sequence tag (EST) library from a key pollinator, the bumblebee Bombus terrestris

Ben M Sadd1*, Michael Kube2, Sven Klages2, Richard Reinhardt2 and Paul Schmid-Hempel1

Author Affiliations

1 Institute of Integrative Biology (IBZ), ETH Zürich, Universitätsstrasse 16, 8092 Zürich, Switzerland

2 Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany

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BMC Genomics 2010, 11:110  doi:10.1186/1471-2164-11-110

Published: 15 February 2010

Additional files

Additional file 1:

Assembly statistics of the Bombus terrestris ESTs.

Format: PDF Size: 56KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Counts of the "best-hits" at the individual taxonomic identifier level for the 13,333 post-assembly Bombus terrestris EST sequences (BLASTX, cutoff = 1e-05).

Format: XLS Size: 42KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Presence and identity of "best-hits" for the 13,333 unique Bombus terrestris sequences in the non-redundant (nr) database of Genbank (version dated 19th October 2009) (BLASTX cutoff = 1e-05).

Format: XLS Size: 2.2MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Gene Ontology assignments for the unique Bombus terrestris sequences.

Format: XLS Size: 2MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 5:

Presence and identity of "best-hits" in paired BLASTX comparisons (cutoff = 1e-05) of the unique Bombus terrestris sequences against proteins from each of Apis mellifera, Nasonia vitripennis, Tribolium castaneum and Drosophila melanogaster.

Format: XLS Size: 3.3MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 6:

Bombus terrestris unique sequences with "hits" to proteins from genes characterised as immune related in Apis mellifera and Drosophila melanogaster.

Format: XLS Size: 68KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data