BNP4 stimulates B-cell development from early bone marrow progenitor cells. Diagrams displaying the cellular composition of cultures as judged by FACS analysis of B220, CD19 and MAC-1 surface expression obtained by in vitro differentiation of 100 LSK cells. The progenitors were incubated for 14 days with either OP9 or NIH3T3 cells and the indicated cytokines. Each dot in the diagrams represents one well and data are collected from at least three independent experiments (n = 13-18). Line indicates the average of all the analyzed wells and the P values has been calculated as compared to the cellular composition obtained on NIH3T3 cells with the addition of FL and IL7.
Zetterblad et al. BMC Genomics 2010 11:108 doi:10.1186/1471-2164-11-108