Differential ability of OP9 and NIH3T3 cells to stimulate B-cell development from hematopoetic progenitor cells. Representative FACS plots of the cells generated after 14 days of incubation of 100 LSK cells on OP9 or NIH3T3 cells with the indicated addition of cytokines. The cells were analyzed with regard to the expression of CD19, B220, and MAC1 as indicated in the diagrams. The numbers in the panels represents average percentages of different cell subsets obtained under the defined conditions after analysis of at least three independent experiments.
Zetterblad et al. BMC Genomics 2010 11:108 doi:10.1186/1471-2164-11-108