Transcript level of AOX and DHAS. RT-PCR was performed on RNA samples using AOX and DHAS primers from glucose containing precultures (OD 2,3), and cultures shifted for 1 or 2 hrs to methanol medium. As loading control transcript levels of actin were analysed.
van Zutphen et al. BMC Genomics 2010 11:1 doi:10.1186/1471-2164-11-1