Validation of microarray differential gene expression data by quantitative real time RT-PCR (qPCR). Ratio of treated vs. control were calculated for microarray data (ordinate) and qPCR data (abscissa). A total of six genes that were up or down regulated with amoxicillin, chlortetracycline or enrofloxacin were compared. The correlation coefficient r2 was 0.84.
Nanduri et al. BMC Genomics 2009 10(Suppl 2):S4 doi:10.1186/1471-2164-10-S2-S4