Table 4

Results of the white-throated sparrow "morph" assay using the DraI restriction site located in the VIP gene [50]. Species heterozygous for the DraI restriction site show 3 fragments (F1-F3); species homozygous for the DraI restriction site show 2 bands, one smaller band at approximately 72–89 bp and one larger band at 185–190 bp (F1 and F2); finally, homozygotes that do not carry the DraI restriction site show only a single band at 284 bp (F3).

Species

Subspecies

Genotype

VIP F1

(bp)

VIP F2

(bp)

VIP F3

(bp)

#

%


White-throated sparrow

(Zonotrichia albicollis)

2m/2m

Homozygous – DraI site

88

189

-

0

0

2m/2 (White Morph)

Heterozygous

88

189

284

278

50.9

2/2 (Tan Morph)

Homozygous – No site

-

-

284

268

49.1


White-crowned sparrow

(Z. leucophyrs)

Homozygous – DraI site

80

190

-

2

16.7

Heterozygous

80

190

284

4

33.3

Homozygous – No site

-

-

284

6

50.0


Golden-crowned sparrow

(Z. atricapilla)

Homozygous – DraI site

83

190

-

0

0

Heterozygous

83

190

284

3

100

Homozygous – No site

-

-

284

0

0


Harris's sparrow

(Z. querula)

Homozygous – DraI site

72/73

88/91

-

1

25.0

Heterozygous

72 or 73

88 or 91

284

0

0

Homozygous – No site

-

-

284

3

75.0


Rufous-collared sparrow

(Z. capensis)

Homozygous – DraI site

89

191

-

10

100


Dark-eyed junco

(Junco hyemalis)

Slate-colored

hyemalis

Homozygous – DraI site

89

189

-

4

100

Oregon

oreganus

Homozygous – DraI site

89

189

-

3

100

Gray-headed

caniceps

Homozygous – DraI site

89

189

-

1

100


Song sparrow

(Melospiza melodia)

Homozygous – DraI site

89

185

-

5

100


Swamp sparrow

(M. georgiana)

Homozygous – DraI site

89

186/189

-

5

100


Purple finch

(Carpodacus purpureus)

Homozygous – DraI site

89

193

-

1

100


Romanov et al. BMC Genomics 2009 10(Suppl 2):S10   doi:10.1186/1471-2164-10-S2-S10

Open Data