MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences

doi:10.1186/1471-2164-10-72

BMC Genomics

Volume 10

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Venier P
De Pittà C
Bernante F
Varotto L
De Nardi B
Bovo G
Roch P
Novoa B
Figueras A
Pallavicini A
Lanfranchi G

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Venier P
De Pittà C
Bernante F
Varotto L
De Nardi B
Bovo G
Roch P
Novoa B
Figueras A
Pallavicini A
Lanfranchi G
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MytiBase: a knowledgebase of mussel (M. galloprovincialis) transcribed sequences

Paola Venier¹^* , Cristiano De Pittà¹^,2^* , Filippo Bernante¹^,2 , Laura Varotto¹ , Barbara De Nardi³ , Giuseppe Bovo⁴ , Philippe Roch⁵ , Beatriz Novoa⁶ , Antonio Figueras⁶ , Alberto Pallavicini⁷ and Gerolamo Lanfranchi¹^,2

¹Department of Biology, University of Padova, Via U Bassi, 58/B, 35121, Padova, Italy

²C.R.I.B.I. Biotechnology Centre, University of Padova, Via U Bassi, 58/B, 35121, Padova, Italy

³Council for Research and Experimentation in Agriculture, Viale XXVIII Aprile, 26, 31015 Conegliano, Treviso, Italy

⁴Institue of Veterinary Sciences (IZSVe), Viale dell'Università, 10, 35020 Legnaro, Padova, Italy

⁵Lagoon Ecosystems UMR 5119, University of Montpellier 2, cc093, place E Bataillon, F-34095 Montpellier cedex 05, France

⁶Institute of Marine Research, CSIC, C/Eduardo Cabello, 6, E-36208 Vigo, Spain

⁷Department of Biology, University of Trieste, P.le Valmaura, 9, 34148 Trieste, Italy

author email corresponding author email* Contributed equally

BMC Genomics 2009, 10:72doi:10.1186/1471-2164-10-72


Published:	9 February 2009

Abstract

Background

Although Bivalves are among the most studied marine organisms due to their ecological role, economic importance and use in pollution biomonitoring, very little information is available on the genome sequences of mussels. This study reports the functional analysis of a large-scale Expressed Sequence Tag (EST) sequencing from different tissues of Mytilus galloprovincialis (the Mediterranean mussel) challenged with toxic pollutants, temperature and potentially pathogenic bacteria.

Results

We have constructed and sequenced seventeen cDNA libraries from different Mediterranean mussel tissues: gills, digestive gland, foot, anterior and posterior adductor muscle, mantle and haemocytes. A total of 24,939 clones were sequenced from these libraries generating 18,788 high-quality ESTs which were assembled into 2,446 overlapping clusters and 4,666 singletons resulting in a total of 7,112 non-redundant sequences. In particular, a high-quality normalized cDNA library (Nor01) was constructed as determined by the high rate of gene discovery (65.6%). Bioinformatic screening of the non-redundant M. galloprovincialis sequences identified 159 microsatellite-containing ESTs. Clusters, consensuses, related similarities and gene ontology searches have been organized in a dedicated, searchable database http://mussel.cribi.unipd.it webcite.

Conclusion

We defined the first species-specific catalogue of M. galloprovincialis ESTs including 7,112 unique transcribed sequences. Putative microsatellite markers were identified. This annotated catalogue represents a valuable platform for expression studies, marker validation and genetic linkage analysis for investigations in the biology of Mediterranean mussels.