Research article
Desiccation survival in an Antarctic nematode: molecular analysis using expressed sequenced tags
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* Corresponding author: Bishwo N Adhikari adhikaribn@hotmail.com
1 Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA
2 Department of Biology and Natural Resource Ecology Laboratory, Colorado State University, Fort Collins, CO, USA
3 Department of Biology and Evolutionary Ecology Laboratories, Brigham Young University, Provo, UT, USA
BMC Genomics 2009, 10:69 doi:10.1186/1471-2164-10-69
Published: 9 February 2009Additional files
Additional File 1:
Distribution of (a) molecular functions, (b) cellular components, and (c) biological process categories based on gene ontology for Plectus murrayi unique sequences. Distribution of (a) molecular functions, (b) cellular components, and (c) biological process categories based on gene ontology for Plectus murrayi unique sequences. More information provided in Figure 2a, b and 2c. Note that individual GO categories can have multiple mappings.
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Additional File 2:
Analysis of mRNA copy number (×107) of Pm-hsp-90: heat shock protein 90 and Pm-hsp-70: heat shock protein 70 gene in Plectus murrayi under desiccated and normal condition. Analysis of mRNA copy number (×107) of Pm-hsp-90: heat shock protein 90 and Pm-hsp-70: heat shock protein 70 gene in Plectus murrayi under desiccated and normal condition. The experiment was performed using an absolute quantitation method of quantitative real-time PCR analysis with each value represents the mean ± SE of three replicates. Nematode samples were exposed to 97 and 85% RH for 3 and 2 days respectively prior to RNA extraction. Controls received no treatment. *Significant difference (P < 0.05) from control.
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Additional File 3:
List of the gene specific primer sequences used for quantitative real-time PCR analysis. List of the gene specific primer sequences used for quantitative real-time PCR analysis. Primers were designed by aligning the EST sequences with their putative homologue from GenBank using IDT SciTools (Integrated DNA Technologies, Coralville, IA, USA) and synthesized by Operon (Operon Biotechnologies Inc., Huntsville, AL, USA).
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