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Open Access Research article

Potential impact of stress activated retrotransposons on genome evolution in a marine diatom

Florian Maumus1, Andrew E Allen12, Corinne Mhiri3, Hanhua Hu1, Kamel Jabbari1, Assaf Vardi14, Marie-Angèle Grandbastien3 and Chris Bowler15*

Author Affiliations

1 CNRS UMR8186, Biologie Moléculaire des Organismes Photosynthétiques, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex05, France

2 J. Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, USA

3 Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA Versailles-Grignon, 78026 Versailles, France

4 Environmental Biophysics and Molecular Ecology Group, Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08901, USA

5 Stazione Zoologica 'Anton Dohrn,' Villa Comunale, I-80121 Naples, Italy

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BMC Genomics 2009, 10:624  doi:10.1186/1471-2164-10-624

Published: 22 December 2009

Abstract

Background

Transposable elements (TEs) are mobile DNA sequences present in the genomes of most organisms. They have been extensively studied in animals, fungi, and plants, and have been shown to have important functions in genome dynamics and species evolution. Recent genomic data can now enlarge the identification and study of TEs to other branches of the eukaryotic tree of life. Diatoms, which belong to the heterokont group, are unicellular eukaryotic algae responsible for around 40% of marine primary productivity. The genomes of a centric diatom, Thalassiosira pseudonana, and a pennate diatom, Phaeodactylum tricornutum, that likely diverged around 90 Mya, have recently become available.

Results

In the present work, we establish that LTR retrotransposons (LTR-RTs) are the most abundant TEs inhabiting these genomes, with a much higher presence in the P. tricornutum genome. We show that the LTR-RTs found in diatoms form two new phylogenetic lineages that appear to be diatom specific and are also found in environmental samples taken from different oceans. Comparative expression analysis in P. tricornutum cells cultured under 16 different conditions demonstrate high levels of transcriptional activity of LTR retrotransposons in response to nitrate limitation and upon exposure to diatom-derived reactive aldehydes, which are known to induce stress responses and cell death. Regulatory aspects of P. tricornutum retrotransposon transcription also include the occurrence of nitrate limitation sensitive cis-regulatory components within LTR elements and cytosine methylation dynamics. Differential insertion patterns in different P. tricornutum accessions isolated from around the world infer the role of LTR-RTs in generating intraspecific genetic variability.

Conclusion

Based on these findings we propose that LTR-RTs may have been important for promoting genome rearrangements in diatoms.