Table 1

Proteins in the epidermis during metamorphosis identified by Mascot searches using protein mass fingerprints (PMF) and MS/MS against the NCBInr_Metazoa Database

Description

PMF

MS/MS


Spot

Protein name

GenBank Accession number and organism

Theoretical MW(kD)/pI

Experimental MW(kD)/pI

Peptide hits

Score*

Coverage

Peptide hits

Score*

Peptide sequences

A

P


22

viral-like chitinase

gi|74422655

[Spodoptera exigua]

61.6/6.14

64/6.55

27

211

38%

-

-

-

-

27

hexamerin

gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.15

17

129

23%

-

-

-

-

28

hexamerin

Gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.21

23

159

26%

-

-

-

-

29

hexamerin

Gi|50404096

[Helicoverpa armigera]

82.0/5.89

75/6.25

25

136

32%

-

-

-

-

36

prophenoloxidase-1

gi|159141810

[Heliothis virescens]

79.1/6.39

67/5.95

11

97

20%

-

-

-

37

arylphorin subunit

gi|5869989

[Spodoptera litura]

76.8/6.7

56/6.75

8

71

12%

2

55

RDPAFYQLYQRI

KDLHQYSYEIIARH

40

heat shock protein 1

gi|56462154

[Lonomia obliqua]

21.9/6.32

16/4.95

9

111

38%

4

230

RNSYFRPWRT

RLLDQHFGMGLKR

RDWWDEWDRPSRL

RHEEKQDEHGFISRQ

42

Phosphoglyceromu-tase

gi|1092224

[Drosophila melanogaster]

28.6/6.62

28/6.1

-

-

-

2

98

RILIAAHGNSLRG

KYGEAQVQIWRR

44

aldehyde

dehydrogenase

gi|164450344

[Ectropis obliqua]

22.0/5.67

(partial)

72/5.89

-

-

-

1

57

KFETFEEVVDRA

45

solute carrier family 25

gi|123232765

[Mus musculus]

55.0/8.81

67/5.55

12

77

24%

-

-

-

46

fructose 1,6-bisphosphate aldolase

gi|45330818

[Antheraea yamamai]

39.7/7.59

44/7.39

12

123

37%

-

-

-

50

large subunit arylphorin p76

gi|27802137

[Heliothis virescens]

30.6/6.33

(partial)

72/6.32

9

91

35%

-

-

-

52

60 S acidic ribosomal

protein P0

gi|18253041

[Spodoptera frugiperda]

34.0/6.34

37/6.35

11

168

53%

-

-

-

55

calponin/transgelin

gi|108875705

[Aedes aegypti]

19.2/7.77

18/7.75

9

121

43%

1

61

KYGVPEEEIFQTADLFERR

58

eukaryotic translation

initiation factor 4A

gi|95102876

[Bombyx mori]

48.0/5.1

53/5.26

29

238

53%

-

-

-

No

59

ATP synthase

beta subunit

gi|115345328

[Bombyx mori]

54.9/5.32

54/5.05

25

236

55%

5

211

RIINVIGEPIDERG

KAHGGYSVFAGVGERT

RVALTGLTVAEYFRD

KVSLVYGQMNEPPGARA

RLVLEVAQHLGENTVRT

No

60

proteasome alpha 3 subunit

gi|114051245

[Bombyx mori]

28. 4/5.27

31/5.3

11

100

30%

2

67

KLYEPGANKRI

KAVENSGTVIGLRG

No

61

heat shock protein 1

gi|56462154

[Lonomia obliqua]

21.9/6.32

27/5.75

7

90

27%

-

-

-

No


Symbols in the table: *, PMF score > 74 significant, MS/MS score > 45 significant; A, protein variation; P, phosphorylation variation; -, not known; ↑, increase compared to 5th-F; ↓ decrease compared to 5th-F; No, no change.

Fu et al. BMC Genomics 2009 10:600   doi:10.1186/1471-2164-10-600

Open Data