MiR-1174 is expressed in An. stephensi, An. gambiae, and Ae. aegypti, but not in T. amboinensis. The top panels are northern results and the bottom panels are RNA gel images for verification of small ribosomal RNA and tRNA integrity and loading of total RNA. Emb, pooled embryos between 0-36 hr after egg deposition; L1+2, pooled 1st and 2nd instar larvae; L3+4, pooled 3rd and 4th instar larvae; Pupae, mixed puape; F, adult females one to five days after emergence; M, adult males one to five days after emergence. 15 μg of total RNA per sample for An. stephensi, An. gambiae, and Ae. aegypti were used. 10 μg of T. amboinensis total RNA per sample were used. For T. amboinensis, 3rd instar larvae were not included. "+ Cont" indicates a positive control which was An. stephensi embryos (12-24 hr).
Li et al. BMC Genomics 2009 10:581 doi:10.1186/1471-2164-10-581