Open Access Highly Accessed Research article

3' tag digital gene expression profiling of human brain and universal reference RNA using Illumina Genome Analyzer

Yan W Asmann1, Eric W Klee1, E Aubrey Thompson2, Edith A Perez2, Sumit Middha1, Ann L Oberg1, Terry M Therneau1, David I Smith3, Gregory A Poland4, Eric D Wieben5 and Jean-Pierre A Kocher1*

Author Affiliations

1 Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, MN 55905, USA

2 Department of Cancer Biology, Mayo Clinic Comprehensive Cancer Center, Mayo Clinic, Jacksonville, FL 32224, USA

3 Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA

4 Mayo Vaccine Research Group, the Program in Translational Immunovirology and Biodefense, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA

5 Advanced Genomics Technology Center DNA sequencing lab, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, USA

For all author emails, please log on.

BMC Genomics 2009, 10:531  doi:10.1186/1471-2164-10-531

Published: 16 November 2009

Additional files

Additional file 1:

Table S1, which is the summary of runs and alignment statistics for all libraries.

Format: XLS Size: 38KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Table S2, a complete table containing the raw gene tag counts (Log 2 scale) and full Pearson correlation coefficient matrix for all lanes and flow cells of all sequencing runs.

Format: XLS Size: 10.2MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Figures S3 - S5 as described in the manuscript.

Format: PPT Size: 203KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Open Data

Additional file 4:

Table S6, representation of expressed ENSEMBL genes in the NGS DGE and Affymetrix U133Plus 2.0 expression data sets.

Format: DOC Size: 26KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Table S7, the number of DpnII sites in each of the Human RefSeq RNAs.

Format: XLS Size: 3.9MB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data