Table 5

Relative gene expression of the reference genes in D. magna after normalisation in the microcystin experiment. D. magna was fed either the green alga S. obliquus or microcystin-free cyanobacteria (S. elongatus or the mutant of M. aeruginosa PCC 7806 [Mut]) or the microcystin-producing wild type of M. aeruginosa PCC 7806 (WT).

Relative expression^a

Gene

S. obliquus

S. elongatus

M. aeruginosa WT

M. aeruginosa Mut

SD^b

alpha-tubulin

1.0000 ± 0.0030

0.3937 ± 0.0021

0.4077 ± 0.0006

0.3028 ± 0.0013

0.3194

TBP

1.0000 ± 0.0094

1.0186 ± 0.0056

1.3176 ± 0.0075

3.0758 ± 0.2210

0.9926

18S

1.0000 ± 0.0015

2.4935 ± 0.0020

1.8613 ± 0.0073

1.0736 ± 0.0002

0.7079

^aValues are mean of n = 3 replicates ± SD. Expression levels are displayed relative to the mean control level

^bSD is the variation of one reference gene across treatments

Schwarzenberger et al. BMC Genomics 2009 10:527   doi:10.1186/1471-2164-10-527