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Open Access Highly Accessed Research article

Transcriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons

Rafael Queiroz12, Corinna Benz13, Kurt Fellenberg4, Jörg D Hoheisel2 and Christine Clayton1*

Author Affiliations

1 Zentrum für Molekulare Biologie der Universität Heidelberg, ZMBH-DKFZ Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany

2 Deutsches Krebsforschungszentrum, In Neuenheimer Feld 280, 69120 Heidelberg, Germany

3 Division of Infection & Immunity and Wellcome Trust Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK

4 Centre for Integrated Protein Sciences Munich (CIPSM), Lehrstuhl für Bioanalytik, Technische Universität München, an der Saatzucht 5, 85354 Freising, Germany

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BMC Genomics 2009, 10:495  doi:10.1186/1471-2164-10-495

Published: 26 October 2009

Abstract

Background

Trypanosome gene expression is regulated almost exclusively at the post-transcriptional level, with mRNA degradation playing a decisive role. When trypanosomes are transferred from the blood of a mammal to the midgut of a Tsetse fly, they transform to procyclic forms: gene expression is reprogrammed, changing the cell surface and switching the mode of energy metabolism. Within the blood, trypanosomes can pre-adapt for Tsetse transmission, becoming growth-arrested stumpy forms. We describe here the transitions in gene expression that occur during differentiation of in-vitro cultured bloodstream forms to procyclic forms.

Results

Some mRNAs showed changes within 30 min of cis-aconitate addition, whereas others responded 12-24 hours later. For the first 12 h after addition of cis-aconitate, cells accumulated at the G1 phase of the cell cycle, and showed decreases in mRNAs required for proliferation, mimicking the changes seen in stumpy forms: many mRNAs needed for ribosomal and flagellar biogenesis showed striking co-regulation. Other mRNAs encoding components of signal transduction pathways and potential regulators were specifically induced only during differentiation. Messenger RNAs encoding proteins required for individual metabolic pathways were often co-regulated.

Conclusion

Trypanosome genes form post-transcriptional regulons in which mRNAs with functions in particular pathways, or encoding components of protein complexes, show almost identical patterns of regulation.