BMC Genomics

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Open Access Research article

A multiway analysis for identifying high integrity bovine BACs

Abhirami Ratnakumar1, Wesley Barris1, Sean McWilliam1, Rudiger Brauning3, John C McEwan3, Warren M Snelling2 and Brian P Dalrymple1*

Author Affiliations

1 CSIRO Livestock Industries, 306 Carmody Road, St. Lucia, QLD 4067, Australia

2 AgResearch, Invermay Agricultural Centre, PB 50034, Mosgiel, New Zealand

3 USDA-ARS-NPA Roman L. Hruska U.S. Meat Animal Res, P.O. BOX 166, Clay Center, NE 6893, USA

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BMC Genomics 2009, 10:46 doi:10.1186/1471-2164-10-46

Published: 23 January 2009

Additional files

Additional file 1:

Table 1. Plates identified as containing BACs with plate-wide systematic identity integrity issues. Rows and/or columns containing putative inconsistent BACs are shown, along with whether the inconsistency is likely to have arisen during fingerprinting or end sequencing. The table also shows the number of BACs on the plate predicted to have systematic identity integrity issues, along with the number of BACs that have been fully sequenced.

Table 2. Plates identified as containing more localised sections of BACs with systematic identity integrity issues. Rows and/or columns containing the putative inconsistent BACs are shown, along with the affected wells, the number of observed affected clones and the number of BACs that have been fully sequenced.

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