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Open Access Highly Accessed Research article

Enrichment of a set of microRNAs during the cotton fiber development

Pieter Bas Kwak1, Qin Qin Wang1, Xu Sheng Chen2, Cheng Xiang Qiu1 and Zhi Min Yang1*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, PR China

2 Laboratory of Cotton Breeding, Industrial Crop Institute, Jiangsu Academy of Agricultural Sciences, Nanjing, PR China

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BMC Genomics 2009, 10:457  doi:10.1186/1471-2164-10-457

Published: 29 September 2009

Additional files

Additional file 1:

Additional Figure S1. Percentage of small RNA sequences with different size derived from wild-type and mutant libraries. All of the reads are of high quality, ranging from 18-28 nt in length.

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Additional file 2:

Additional Table S2: Identified known candidate miRNAs from Gossypium hirsutum wild-type (WT) and fuzz/lintless mutant (M) ovules. "Sequence" stands for the most occurring read that is homologue to the known miRNA.

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Additional file 3:

Additional Table S3. Overlaps of miRNAs between the previously published computational miRNA predictions and those found in this study.

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Additional file 4:

Additional Table S4: Predicted targets for identified candidate miRNAs. miRNAs targeting the same gene and site are grouped together. The first listed miRNA has the least mismatches. "Mm" stands for the total amount of mismatches between the first mentioned miRNA and the predicted target. "Alignment" visually represents miRNA/mRNA complementary base-pairs and mismatches for the first listed miRNA, with vertical bars and spaces as Watson-Crick base-pairs and mismatches, respectively (G:U wobbles count as mismatches).

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