ASE using Solexa. Summary of sample preparation: (a) The forward primer is designed to anneal 1 bp upstream of the known SNP. The 5' tail contains a unique barcode and adapter sequences necessary for hybridization to the Solexa sequencing platform. (b) Model of target enrichment and allele-specific expression represented as sequencing coverage per allele.
Main et al. BMC Genomics 2009 10:422 doi:10.1186/1471-2164-10-422