Table 1

miRNA targeting of neural progenitor maintenance genes

Target Gene


miRNA ID

Fgf2

Fgfr1

Efnb1

Rgs3

Notch1

Mef2d


mmu-let-7 family

(2..8) CUACCUC

mmu-miR-15b and 16

(1..7) GCUGCUA × 2

(2..8) UGCUGCU

(1..7) GCUGCUA

mmu-miR-17 and 20a

(2..8) GCACUUU

mmu-miR-25

(2..8) GUGCAAU

(2..7) UGCAAU

(2..8) GUGCAAU

mmu-miR-30c

(1..7) GUUUACA

mmu-miR-103

(1..8) AUGCUGCU

(2..7) UGCUGC

(2..8) AUGCUGC

mmu-miR-124

(2..8) GUGCCUU

mmu-miR-125 family

(1..7) UCAGGGA


Evolutionarily conserved sites complementary to seed regions of highly expressed miRNAs (among top 30 most expressed) were found in 3'UTRs of the genes previously shown to be involved in maintenance of neural progenitors: Fgf2 - fibroblast growth factor 2 [35], Fgfr1 - fibroblast growth factor receptor 1 [35], Efnb1 - ephrin B1 [36], Rgs3 - PDZ-RGS3 [36], Notch1 [34] and Mef2d - myocyte enhancer factor 2D [70]. RNA sequences in uppercase represent the seed matching sites (from TargetScan [21]) of aligned 3'UTRs. All sequences are conserved between human, mouse, rat and dog. Numbers in parentheses show the the positions of mature miRNA sequences to which the 3'UTR sites are complementary.

Manakov et al. BMC Genomics 2009 10:419   doi:10.1186/1471-2164-10-419

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