Expression levels of miRNAs corresponding to the 10 most depleted or 10 most enriched seed matching sites. We assessed the expression levels of miRNAs with seed-regions complementary to the 10 most depleted (red line) or 10 most enriched words (green line) in 3'UTRs of 17333 profiled genes. Genes are sorted according to average expression (from highest to lowest). The p. values are plotted with a positive sign if the subset of miRNAs is expressed higher than the average level of miRNA expression, and with a negative sign if lower. The subset of miRNAs with seeds complementary to the 10 most depleted words in the beginning of the sorted list (i.e. in 3'UTRs of highly expressed genes) were on average highly expressed (A, red line). If seed matching sites of 12 miRNAs steady-state highly expressed miRNAs (Figure 2) were subtracted, the derived subset of miRNAs was expressed at the average level (B, red line). miRNAs corresponding to the 10 most enriched words (green line) in the second half of the list (i.e. in 3'UTRs of relatively lowly expressed genes) were highly expressed (A, green line). This did not depend on the subtraction of seed matching sites of 12 highly expressed miRNAs (B, green line). Black dotted lines are showing p. value cutoff -log10(0.01) for higher than average expression and log10(0.01) for lower than average expression.
Manakov et al. BMC Genomics 2009 10:419 doi:10.1186/1471-2164-10-419