Distribution of sites complementary to profiled miRNA seed regions. We analyzed distributions of words complementary to the seed regions of profiled miRNAs (positions 2 - 8 of mature sequences) in 3'UTRs of 17,333 profiled genes. Genes are sorted according to the average expression (from highest expressed to lowest expressed). Probability values are plotted with a positive sign for enrichment and with negative sign for depletion. Six out of seven most significantly depleted words in 3'UTRs profiled are shown in shades of red. The most significant depleted word "GTGCCTT" is complementary to positions 2 - 8 of mmu-miR-124. The second most depleted word has a central 5-mer "TGCCT" which is complementary to positions 3 - 7 of mmu-miR-124. The central 5-mer of the third most depleted word is complementary to positions 2 - 6 of both mmu-miR-125a-5p and mmu-miR-125b-5p. The fourth most depleted word "CTACCTC" is complementary to positions of 2 - 8 of eight miRNAs of mmu-let-7 family. The sixth most depleted word contains a 6-mer complementary to positions 3 - 8 of mmu-miR-137, and the seventh to positions 2 - 8 of miR-128.
Manakov et al. BMC Genomics 2009 10:419 doi:10.1186/1471-2164-10-419