Additional file 3.
Figure S1. Differential expression of CD114/CSF3R, CD115/CSF1R, CD93/C1qR1 and C3aR1 on CD14highCD16-, CD14highCD16+, and CD14lowCD16+ monocytes. Freshly isolated PBMC were stained with FITC CD14, PE-Cy5 CD16, and PE CD114, PE CD115, and PE CD93 Abs. The expression of CD3aR1 was detected after staining with unconjugated mouse C3AR1 Ab and PE rat anti-mouse Ab (RAM). CD14highCD16neg (R2), CD14highCD16+ (R3) and CD14lowCD16+ (R4) Mo (A) were analyzed for expression of CD114, CD115, CD93 and C3aR1 (B). Shown is an overlay histogram from one representative donor of 4 donors examined (B, left panels) and graphs showing mean ± SEM for % or MFI of CD114, CD115, CD93, and C3aR1 expression on each Mo subset (B, right panels). (*, Paired t-test p-values < 0.05, CD16+ versus CD16- Mo; n = 4).
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Ancuta et al. BMC Genomics 2009 10:403 doi:10.1186/1471-2164-10-403