Real-time RT-PCR validation of microarray results. The expression of CD16, C3AR1, C1QR1, ICAM-2, CSFR1, CSF3R, CDKN1C, TNFRSF8, and LTB mRNA was quantified by SYBR Green real time RT-PCR in CD16+ and CD16- Mo. The concentration of each gene was normalized to the 28S rRNA internal control and expressed as fgs RNA of a target gene per 1 ng rRNA28S. Depicted are results (mean ± SD of triplicate wells; *, p < 0.05, unpaired t-test, CD16+ versus CD16- Mo) obtained with matched cells from 2 different healthy donors.
Ancuta et al. BMC Genomics 2009 10:403 doi:10.1186/1471-2164-10-403