Table 2

Microarray expression data for sulfur induced genes

ID NC011761

Gene

Function

log₂ ratio median

One sample

t-Test

(p-value)

Proteomic data: strain/references

pet II operon

AFE_2732

hip

High potential iron-sulfur protein

-1,8

0,00*

AFE_2731

petC2

ubiquinol-cytochrome c reductase, cytochrome c1 subunit

-0,3

0,09

AFE_2730

petB2

ubiquinol-cytochrome c reductase, cytochrome b subunit

-1,7

0,00*

AFE_2729

petA2

ubiquinol-cytochrome c reductase, iron-sulfur subunit

-1,9

0,00*

AFE_2728

sdrA2

oxidoreductase, short-chain dehydrogenase/reductase family

-1,1

0,00*

AFE_2727

cycA2

cytochrome c4

-0,5

0,17

Heterodisulfide reductase complex operon

AFE_2586

hdrB

heterodisulfide reductase subunit B, homolog

-1,5

0,00*

AFE_2558

rhd

rhodanese-like domain protein

0,1

0,38

AFE_2557

tusA

conserved hypothetical protein

-2,4

0,00*

AFE_2556

dsrE

conserved hypothetical protein

-2,1

0,00*

AFE_2555

hdrC

iron-sulfur cluster-binding protein

-2,1

0,00*

AFE_2554

hdrB

heterodisulfide reductase subunit B, homolog

-2,0

0,00*

AFE_2553

hdrA

pyridine nucleotide-disulfide oxidoreductase

-2,6

0,00*

AFE_2552

orf2

conserved hypothetical protein

ND

ND

AFE_2551

hdrC

iron-sulfur cluster-binding protein

-2,4

0,00*

AFE_2550

hdrB

succinate dehydrogenase/fumarate reductase, C subunit

-1,9

0,00*

Sulfide-quinone reductase

AFE_1792

sqr

sulfide-quinone reductase, putative

-1,6

0,00*

CCM 4253/[24]

NASF-1/[26]

Cytochrome bd ubiquinol oxidase

AFE_0955

cydA

cytochrome d ubiquinol oxidase, subunit I

-2,0

0,00*

AFE_0954

cydB

cytochrome d ubiquinol oxidase, subunit II

-2,6

0,00*

Cytochrome bo3 ubiquinol oxidase

AFE_0634

cyoD

cytochrome o ubiquinol oxidase, subunit IV

-2,3

0,00*

AFE_0633

cyoC

cytochrome o ubiquinol oxidase, subunit III

-3,0

0,00*

AFE_0632

cyoB

cytochrome o ubiquinol oxidase, subunit I

-2,7

0,00*

AFE_0631

cyoA

cytochrome o ubiquinol oxidase, subunit II

-3,2

0,00*

Sulfate adenylyltransferase

AFE_0539

sat

sulfate adenylyltransferase, putative/adenylylsulfate kinase

0,7

0,00*

Thiosulfate-quinone oxidoreductase complex operon

AFE_0046

conserved hypothetical protein

-1,7

0,00*

AFE_0045

sulfur/pyrite/thiosulfate/sulfide-induced protein

-1,1

0,00*

ATCC 19859/[25]

ATCC 19859/[28]

AFE_0044

doxDA

Thiosulfate-quinone oxidoreductase, DoxD-like family protein

-2,3

0,00*

AFE_0043

periplasmic solute-binding protein, putative

-2,2

0,00*

CCM 4253/[24]

ATCC 23270/[25]

ATCC 23270/[29]

AFE_0042

Tat pathway signal sequence domain protein

-1,5

0,00*

AFE_0041

C4-dicarboxylate transporter/malic acid transport protein

-1,5

0,00*

Tetrathionate hydrolase

AFE_0029

tetH

Tetrathionate hydrolase

-0,6

0,22*

ATCC 23270/[27]

Gene expression values (log₂ ratio of median) for all genes/operons alluded in the revised model of sulfur oxidation in A. ferrooxidans ATCC 23270. Genes with a log₂ ratio of median larger than |1.5| (corresponding to genes induced more than 2.8 fold) are considered differentially expressed (indicated with *) and genes p-value <0,005 are considered significant. Gene ID is that of Genbank genome annotation NC 011761. The reference and the strain in which the level of the gene product has been shown to be higher in S⁰ than in Fe(II) conditions are indicated in the last column.

Quatrini et al. BMC Genomics 2009 10:394   doi:10.1186/1471-2164-10-394