Experimental Design. Mouse bone marrow derived monocytes were cultured in the presence of CSF1 conditioned medium in six well plates for 7 days in order to allow differentiation into macrophages (BMDM). A series of control transfections were performed to assess the effect of Lipofectamine2000 and control RISC-Free siRNA. Six independent wells (on different plates) were then treated with either siRNAs targetting the mRNAs of one of 11 genes of interest or control siRNAs. 24 hours later three wells of each siRNA treament were stimuated by IFNγ and the cells were harvested 24 hours later. Total RNA was extracted and 150 ng labelled using whole transcript labelling and the samples run on Affymetrix mouse exon arrays. Data was then subjected to both network and statistical analyses.
Lacaze et al. BMC Genomics 2009 10:372 doi:10.1186/1471-2164-10-372