Crosstalk between IL-6 and gangliosides in hEBD LVEC cells. (A) IL-6 mRNA decreased upon supplementation of the culture medium with 5.0 μM of either GM3 or GD3 (n ≥ 3; p < 0.01 for all time points shown compared with controls that were incubated in the absence of IL-6). (B) Ganglioside GM3 decreased in a dose-dependent manner in cells supplemented with 1.0 or 4.0 ng/ml IL-6 for 24 h. (C) mRNA levels for IL-6 and TLR-4 increased in cells incubated with IL-6 (error bars represent SD for n ≥ 3 independent experiments and p < 0.05 for both measurements).
Wang et al. BMC Genomics 2009 10:356 doi:10.1186/1471-2164-10-356