Table 3 |
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|
Q-PCR primers with RSq and efficiency values |
||||
|
Gene Name |
Clone ID |
Primer Sequence |
RSq |
% Efficiency |
|
|
||||
|
Housekeeping sequence |
||||
|
|
||||
|
Unknown |
sb_009_01G07 |
F: CTCGGACTCAGCCTGTCTA R: ATAAATCGGACTTCCATTTCCA |
0.982 |
95 |
|
|
||||
|
|
||||
|
Aquaporins |
||||
|
|
||||
|
AQP-A |
sb_006_02P03 |
F: ATGTTGTAGGAGGAAGCGTCA R: ACAAACCGTCGTCTCGGTAG |
0.991 |
115 |
|
|
||||
|
AQP-B |
sb_006_05H07 |
F: ATTGGTGCTTGGTTGTTGAA R: AATCCTGCTCCAGTGAATCC |
0.996 |
98 |
|
|
||||
|
AQP-C |
sb_006_08O08 |
F: TACCGTCGCTTCCATGATTA R: CCCTTCACGTAGCTCCAGTT |
0.995 |
105 |
|
|
||||
|
|
||||
|
Heat shock proteins |
||||
|
|
||||
|
sHSP |
sb_009_11D21 |
F: ACTCCACGCCAGCATTTCTTC R: TCCAATTCTGTCCGCATTATTCC |
0.991 |
111 |
|
|
||||
|
HSP70 |
sb_009_03K12 |
F: CAAGGAGGTGGACAACAACAATC R: CACAATCATTCAGCAGCAATAACAC |
0.994 |
92 |
|
|
||||
|
|
||||
|
Antioxidants |
||||
|
|
||||
|
Superoxide dismutase |
sb_009_01L22 |
F: ACGAGAAGGTTGACGATTTGGG R: ATTCCGCAGCCTAAACGAGAC |
0.998 |
86 |
|
|
||||
|
Catalase |
sb_006_03D21 |
F: CCCGAGATGGATTTATGAGTCC R: CTAAGTACACAGAAGCCACACC |
0.991 |
183 |
|
|
||||
|
Glutathione-S-transferase |
sb_006_04F01 |
F: TGCTCCAAGTCGTGCCGTTC R: GATGGCTCGTGACTCGCTTAG |
0.988 |
153 |
|
|
||||
|
|
||||
|
Various |
||||
|
|
||||
|
Ferritin |
sb_006_04H15 |
F: GGAGGTCGTGTCGTTCTTCAG R: CAGTTGTGGGTCACCATTTCG |
0.991 |
94 |
|
|
||||
|
Desaturase |
sb_006_09F19 |
F: GCTCCTGACCCGTAAACATCC R: CCATGCTGCCAATAAACTTTCACC |
0.996 |
105 |
|
|
||||
|
Trehalose GO annotations |
||||
|
|
||||
|
Trehalose 6 phosphate synthase (1) |
sb_006_04P17 |
F: TGAATTGGACGATTACGCTGAAG R: AGACTGCCCATTGCTTTGAGG |
1.00 |
86 |
|
|
||||
|
Trehalose 6 phosphate synthase (2) |
sb_006_06G01 |
F: GGATGGAATTACTGGAGCTTGG R: GTGCTTGATGAGCTGTGAAACC |
0.985 |
110 |
|
|
||||
|
LATS tumour supressor |
sb_006_04E02 |
F: CGGGACGGACATATCAAACT R: TGAGCAAGGCATCGTACATT |
0.995 |
97 |
|
|
||||
|
Serine/threonine protein kinase 38 |
sb_006_04N08 |
F: GACTGGTGGAGTCTGGGAGT R: CAGTCCACGCTTTTGAAGAA |
0.992 |
122 |
|
|
||||
|
Protein kinase A cAMP dependant catalytic sub-unit |
sb_006_07A12 |
F: TCAAAGGTCGAACCTGGACT R: AACCGTACTTTCCCTGCAAC |
0.990 |
100 |
|
|
||||
|
Trehalase precursor |
sb_006_01B14 |
F: CCGTAGATGGACTTCCTGGT R: TGCCTGTCAGAACACACAAA |
0.985 |
117 |
|
|
||||
|
P70 ribosomal protein S6 kinase |
sb_006_01P13 |
F: GGGCGAGATGCTAATGAAAT R: CCTTCGTGTGAGCAGTGTCT |
0.991 |
115 |
|
|
||||
|
Putative protein kinase DC2 |
sb_006_05C18 |
F: ATGGAGAATGGCACTGAGGAC R: CTTAGGCGTCTTTGGTAAACATCC |
0.995 |
99 |
|
|
||||
|
Similar: serine/threonine protein kinase 6 (Aurora family kinase 1) |
sb_006_07A19 |
F: ACTTTGACATTGGGCGTCTC R: CACCAGGGGCATATTCAAGA |
0.934 |
180 |
|
|
||||
|
Protein kinase C |
sb_008_03M09 |
F: ACTCCACGATGATGTGTTGTATCC R: TCCAGAACCACTTCCTTGATTGC |
0.995 |
80 |
|
|
||||
|
cAMP dependant protein kinase C1 |
sb_009_02D08 |
F: CAACGTCATCTACCGTGACC R: AAATACTCCGGTGTCCCACA |
0.988 |
102 |
|
|
||||
|
Clark et al. BMC Genomics 2009 10:328 doi:10.1186/1471-2164-10-328 |
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