BMC Genomics

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Open Access Highly Access Research article

Surviving the cold: molecular analyses of insect cryoprotective dehydration in the Arctic springtail Megaphorura arctica (Tullberg)

Melody S Clark1*, Michael AS Thorne1, Jelena Purać1,2, Gavin Burns1, Guy Hillyard1, Željko D Popović2, Gordana Grubor-Lajšić2 and M Roger Worland1

Author Affiliations

1 British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK

2 University of Novi-Sad, Faculty of Sciences, Trg Dositeja Obradovića 3, 21000 Novi Sad, Republic of Serbia

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BMC Genomics 2009, 10:328 doi:10.1186/1471-2164-10-328

Published: 21 July 2009

Additional files

Additional file 1:

The "Top 20" sequenced up-regulated clones in the -2°C cold dehydration experiment, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. Definitions: LogFold = Estimate of the log2-fold change corresponding to the effect or contrast; AveExpr = Average log2 expression for the probe over all arrays and channels; adj p val = adjusted as described in methods; B = log odds that the gene is differentially expressed. BLAST sequence similarity data.

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Additional file 2:

The "Top 20" sequenced up-regulated clones in the -7°C cold dehydration experiment, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. Detail of columns: as for Additional file 1. BLAST sequence similarity data.

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Additional file 3:

The "Top 20" sequenced up-regulated clones in the 0.9 salt dehydrated experiment, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. Detail of columns: as for Additional file 1. BLAST sequence similarity data.

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Additional file 4:

The "Top 20" sequenced up-regulated clones in the 0.2 salt dehydrated experiment, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. Detail of columns: as for Additional file 1. BLAST sequence similarity data.

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Additional file 5:

The "Top 20" sequenced up-regulated clones in the 18 hour recovery experiment, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. Detail of columns: as for Additional file 1. BLAST sequence similarity data.

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Additional file 6:

Clones co-regulated with TPS across all four dehydration treatments, with putative functionality assigned via BLAST sequence similarity searching. All matches are in excess of 1.0 e-10 unless stated in the discussion. BLAST sequence similarity data.

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Open Data