Open Access Highly Accessed Research article

A comparison of RNA amplification techniques at sub-nanogram input concentration

Julie E Lang15*, Mark Jesus M Magbanua2, Janet H Scott2, G Mike Makrigiorgos4, Gang Wang4, Scot Federman2, Laura J Esserman1, John W Park2 and Christopher M Haqq23

Author Affiliations

1 Department of Surgery, UCSF Comprehensive Cancer Center, 1500 Divisadero Street, San Francisco, CA 94143, USA

2 Department of Medical Oncology, UCSF Comprehensive Cancer Center, San Francisco, CA 94143, USA

3 Department of Urology, UCSF Comprehensive Cancer Center, San Francisco, CA 94143, USA

4 Dana Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA

5 Department of Surgery, Arizona Cancer Center, University of Arizona, 1515 N, Campbell Ave #1968, PO Box 245024, Tucson, AZ 85724, USA

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BMC Genomics 2009, 10:326  doi:10.1186/1471-2164-10-326

Published: 20 July 2009

Additional files

Additional file 1:

Table S2. QPCR genes.

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Additional file 2:

Figure S1: Dynamic Range of QPCR Probes for BT474 Versus StratRef. The delta delta CT of our QPCR probes covered a dynamic range of negative 21 to positive 9 and were selected without bias towards any of the amplification techniques.

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Additional file 3:

Table S1-8. SAM Analysis.

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