Table 1

ExpressSeq read counts that hit* NCBI databases

TSEQ

TSEQ

ODT

ODT

MAQC A

MAQC B

MAQC A

MAQC B


Total # of Reads

823,575

1,079,508

881,555

846,304

Reads that Hit Human Genomic DNA

89%

96%

97%

96%

Reads that Hit Human RefSeq (38,698 sequences)

60%

58%

73%

67%

Reads that Hit Human NM's (24,654 sequences)

53%

50%

59%

45%

Reads that Hit CCDS (17,751 sequences)

31%

23%

35%

20%

Reads that Hit AceView (278,760 sequences)

77%

85%

83%

84%

Orphan Reads (Hit none of the above)

10%

4%

3%

4%


*An ExpressSeq "hit" is the single best BLAST alignment of the GS FLX reads to sequences in each database with e ≤ 10-20.

Mane et al. BMC Genomics 2009 10:264   doi:10.1186/1471-2164-10-264

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