Figure 3.

Strand-specific RT-PCR for target Carbonic anhydrase (Ta.5227.1). Sense-strand transcription was detected only for germinated seed tissue, and both sense- and antisense-strand transcription for all other tissues. M: DNA marker, 1: Germinated seed antisense, 2: Germinated seed sense, 3: Shoot antisense, 4: Shoot sense, 5: Flag leaf antisense, 6: Flag leaf sense, 7: Spike pre-anthesis antisense, 8: Spike pre-anthesis sense, 9: Spike post-anthesis antisense, 10: Spike post-anthesis sense, N: Negative controls.