Table 1 |
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RNA Extraction Strategy. |
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Pre-adult stages |
Adult Stages |
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Exposed to short days to induce diapause development |
Exposed to long days to induce direct development |
Non-reproductive Fed only sugar |
Reproductive Fed sugar and protein (liver) |
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First Instar Larvae |
First Instar Larvae |
Day 1 Males |
Day 1 Males |
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Second Instar Larvae |
Second Instar Larvae |
Day 1 Females |
Day 1 Females |
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Early Third Instar Larvae |
Early Third Instar Larvae |
Day 2 Males |
Day 2 Males |
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Late Third Instar Larvae |
Late Third Instar Larvae |
Day 2 Females |
Day 2 Females |
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Wandering Larvae |
Wandering Larvae |
Day 3 Males |
Day 3 Males |
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Pupae |
Early Diapause Pupae (15 days) |
Day 3 Females |
Day 3 Females |
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Early Pharate Adult |
Mid-diapause Pupae (30 days) |
Day 4 Males |
Day 4 Males |
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Mid-Pharate Adult (red eye stage) |
Late Diapause Pupae (60 Days) |
Day 4 Females |
Day 4 Females |
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Late Pharate Adult (black bristle stage) |
Day 5 Males |
Day 5 Males |
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Day 5 Females |
Day 5 Females |
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Day 7 Males |
Day 7 Males |
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Day 7 Females |
Day 7 Females |
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Day 9 Males |
Day 9 Males |
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Day 9 Females |
Day 9 Females |
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Flesh fly life stages that were individually RNA extracted and combined equally prior to cDNA library synthesis and normalization. |
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Hahn et al. BMC Genomics 2009 10:234 doi:10.1186/1471-2164-10-234 |
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