Table 1

RNA Extraction Strategy.

Pre-adult stages

Adult Stages


Exposed to short days to induce diapause development

Exposed to long days to induce direct development

Non-reproductive

Fed only sugar

Reproductive

Fed sugar and protein (liver)

First Instar Larvae

First Instar Larvae

Day 1 Males

Day 1 Males

Second Instar Larvae

Second Instar Larvae

Day 1 Females

Day 1 Females

Early Third Instar Larvae

Early Third Instar Larvae

Day 2 Males

Day 2 Males

Late Third Instar Larvae

Late Third Instar Larvae

Day 2 Females

Day 2 Females

Wandering Larvae

Wandering Larvae

Day 3 Males

Day 3 Males

Pupae

Early Diapause Pupae (15 days)

Day 3 Females

Day 3 Females

Early Pharate Adult

Mid-diapause Pupae (30 days)

Day 4 Males

Day 4 Males

Mid-Pharate Adult (red eye stage)

Late Diapause Pupae (60 Days)

Day 4 Females

Day 4 Females

Late Pharate Adult (black bristle stage)

Day 5 Males

Day 5 Males

Day 5 Females

Day 5 Females

Day 7 Males

Day 7 Males

Day 7 Females

Day 7 Females

Day 9 Males

Day 9 Males

Day 9 Females

Day 9 Females


Flesh fly life stages that were individually RNA extracted and combined equally prior to cDNA library synthesis and normalization.

Hahn et al. BMC Genomics 2009 10:234   doi:10.1186/1471-2164-10-234

Open Data