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Open Access Highly Accessed Research article

Gene discovery using massively parallel pyrosequencing to develop ESTs for the flesh fly Sarcophaga crassipalpis

Daniel A Hahn1*, Gregory J Ragland1, D DeWayne Shoemaker2 and David L Denlinger3

Author Affiliations

1 Department of Entomology and Nematology, The University of Florida, PO Box 110620, Gainesville, Fl, 32611-0620, USA

2 USDA ARS, Center for Medical, Agricultural, and Veterinary Entomology, 1600 SW 23rd Drive, Gainesville, FL 32608, USA

3 Department of Entomology, Ohio State University, 400 Aronoff Laboratory, 318 West 12th Avenue, Columbus, OH 43210, USA

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BMC Genomics 2009, 10:234  doi:10.1186/1471-2164-10-234

Published: 19 May 2009

Additional files

Additional file 1:

Examples of potential diapause-related cDNAs identified in this EST library.

Format: PDF Size: 80KB Download file

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Open Data

Additional file 2:

Microsatellite discovery. 2a. Summary of potential microsatellite loci identified. 2b. List of contigs and singletons containing potential microsatellite loci including repeat type and length.

Format: PDF Size: 204KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data