Research article
Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx
1 University of Texas at Austin, 1 University Station C0930, Austin, TX, 78712, USA
2 The Center for Genomics and Bioinformatics, Indiana University, 915 East Third Street, Bloomington, IN, 47405, USA
3 ARC Centre of Excellence for Coral Reef Studies, and School of Marine and Tropical Biology, James Cook University, Townsville, QLD, 4811, Australia
BMC Genomics 2009, 10:219 doi:10.1186/1471-2164-10-219
Published: 12 May 2009Additional files
Additional file 1:
Validation of singleton sequences and contig joining procedure by PCR amplification. This document contains gel photographs of the PCR products obtained from validation of randomly-selected scaffold and singleton sequences.
Format: DOC Size: 1.7MB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 2:
Effects of sequence length on the proportion of sequences for which significant matches were found. This PDF file contains a bar plot summarizing the effects of sequence length on the proportion of sequences for which significant blast matches were found.
Format: PDF Size: 4KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 3:
Validation of predicted SNPs by PCR and Sanger sequencing. The table in this document shows the primer sequences used to amplify and sequence each of the 20 SNPs selected for validation, as well as the different alleles detected for each.
Format: DOC Size: 61KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 4:
Detailed protocol for preparing cDNA for 454 transcriptome sequencing. This document gives a step-by-step protocol for preparing cDNA for 454 sequencing.
Format: DOC Size: 273KB Download file
This file can be viewed with: Microsoft Word Viewer
