Open Access Highly Accessed Research article

Water deficit alters differentially metabolic pathways affecting important flavor and quality traits in grape berries of Cabernet Sauvignon and Chardonnay

Laurent G Deluc1, David R Quilici1, Alain Decendit2, Jérôme Grimplet1, Matthew D Wheatley1, Karen A Schlauch1, Jean-Michel Mérillon2, John C Cushman1 and Grant R Cramer1*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, Mail Stop 200, University of Nevada, Reno, Nevada 89557, USA

2 Groupe d'Études des Substances Végétales à Activité Biologique, EA 3675, Institut des Sciences de la Vigne et du Vin, Université Victor Segalen Bordeaux 2, UFR Sciences Pharmaceutiques, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France

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BMC Genomics 2009, 10:212  doi:10.1186/1471-2164-10-212

Published: 8 May 2009

Additional files

Additional file 1:

Forward and reverse primers used with qRT-PCR. This file provides a list of all of the forward and reverse primers used with qRT-PCR for the estimation of relative transcript abundance.

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Additional file 2:

Linear regression of the transcript abundance values of the qRT-PCR with the corresponding values of the same transcript on the microarray. qRT-PCR data are on the y-axis and the microarray data are on the x-axis. Each value represents the mean of 6 different time points for each transcript. Data were normalized to the transcript abundance of an ankyrin-repeat protein (1612584_s_at) that did not change with developmental stage or stress treatment. Ferulate-5-hydroxylase (F5H, 1614502_at, TC63764) is represented by solid grey squares (Cabernet Sauvignon with well-watered conditions; CS-WW) and by red triangles (Cabernet Sauvignon with water deficit; CS-WD). 9-cis-epoxycarotenoid dioxygenase (NCED1, 1608022_at, TC57089) is represented by orange triangles (CS-WW) and orange diamonds (CS-WD). Expansin (1607674_at, TC54149) is represented by solid green circles (Chardonnay with well-watered conditions; CH-WW) and open light blue triangles (CH-WD). Diacyl glycerol kinase 2 (1618308_at, TC63661) is represented by open green squares (CH-WW) and open dark blue triangles (CH-WD).

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Additional file 3:

Probe sets that exhibited a significant Treatment effect in their expression levels. The list of probe sets was selected based upon statistical analysis.

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Additional file 4:

Probe sets that exhibited a significant Cultivar and Treatment effect. The list of probe sets was selected based upon statistical analysis.

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Additional file 5:

Probe sets that exhibited a significant Cultivar, Treatment, and Time interaction effect. The list of probe sets was selected based upon statistical analysis.

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Additional file 6:

Post-RMA Perfect Match (PM) intensity boxplots of all 84 Vitis Genome Arrays. This is a plot to assess the quality of the data from the arrays.

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Additional file 7:

Post-RMA Perfect Match (PM) intensity distributions of all 84 Vitis Genome Arrays. This is a plot to assess the quality of the data from the arrays.

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Additional file 8:

RNA digestion plot of all 84 Vitis Genome Arrays. This is a plot to assess the quality of the data from the arrays.

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Additional file 9:

A representative HPLC chromatogram for carotenoid identification and quantification. This is a plot to assess the quality of the data and the retention times from the chromatogram.

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