An integrated RH map of porcine chromosome 10
1 Department of Dairy and Animal Science, College of Agricultural Sciences, Pennsylvania State University, 305 Henning Building, University Park, PA, USA
2 Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science & Technology, Xi'an Jiaotong University, Xi'an, PR China
3 Genome Research Department, National Institute of Agrobiological Sciences, Ikenodai, Tsukuba, Ibaraki 305-0901, Japan
4 Department of Biology, College of Sciences, University of Nevada, Reno, NV, USA
5 Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan
6 Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, IL, USA
7 Department of Surgical Oncology, Room 618 820 CSB, University of Illinois COM, 840 South Wood St, Chicago, IL, USA
BMC Genomics 2009, 10:211 doi:10.1186/1471-2164-10-211Published: 8 May 2009
Whole genome radiation hybrid (WG-RH) maps serve as "scaffolds" to significantly improve the orientation of small bacterial artificial chromosome (BAC) contigs, order genes within the contigs and assist assembly of a sequence-ready map for virtually any species. Here, we report the construction of a porcine: human comparative map for pig (Sus scrofa) chromosome 10 (SSC10) using the IMNpRH212,000-rad porcine WG-RH panel, integrated with the IMpRH7000-rad WG-RH, genetic and BAC fingerprinted contig (FPC) maps.
Map vectors from the IMNpRH212,000-rad and IMpRH7,000-rad panels were merged to construct parallel framework (FW) maps, within which FW markers common to both panels have an identical order. This strategy reduced map discrepancies between the two panels and significantly improved map accuracy. A total of 216 markers, including 50 microsatellites (MSs), 97 genes and ESTs, and 69 BAC end sequences (BESs), were ordered within two linkage groups at two point (2 pt) LOD score of 8. One linkage group covers SSC10p with accumulated map distances of 738.2 cR7,000 and 1814.5 cR12,000, respectively. The second group covers SSC10q at map distances of 1336.9 cR7,000 and 3353.6 cR12,000, yielding an overall average map resolution of 16.4 kb/cR12,000 or 393.5 kb per marker on SSC10. This represents a ~2.5-fold increase in map resolution over the IMpRH7,000-rad panel. Based on 127 porcine markers that have homologous sequences in the human genome, a detailed comparative map between SSC10 and human (Homo sapiens) chromosome (HSA) 1, 9 and 10 was built.
This initial comparative RH map of SSC10 refines the syntenic regions between SSC10 and HSA1, 9 and 10. It integrates the IMNpRH212,000-rad and IMpRH7,000-rad, genetic and BAC FPC maps and provides a scaffold to close potential gaps between contigs prior to genome sequencing and assembly. This map is also useful in fine mapping of QTLs on SSC10.