Research article
Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery
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* Corresponding author: Matthew C Hale mchale@purdue.edu
1 Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN 47907, USA
2 Cornell Biological Field Station, 900 Shackelton Point Road, Bridgeport, NY 13030, USA
BMC Genomics 2009, 10:203 doi:10.1186/1471-2164-10-203
Published: 29 April 2009Additional files
Additional File 1:
Differences in expression of genes between normalized and native libraries (Molecular Function categories). List of genes found within the Molecular function category of the Gene Ontology assignment with a significant difference in expression between normalized and native libraries.
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Additional file 2:
Differences in expression of genes between normalized and native libraries (Biological Process categories). List of genes found within the Biological Process category of the Gene Ontology assignment.
Format: DOC Size: 113KB Download file
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Additional file 3:
Differences in expression of genes between normalized and native libraries (Cellular Component categories). List of genes found within the Cellular Component category of the Gene Ontology assignment.
Format: DOC Size: 108KB Download file
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Additional file 4:
Details of all SNPs detected in contigs. List of contigs with associated contig length, contig depth, number of SNPs and Ts/Tv ratio.
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Additonal File 5:
Details of all SNPs in contigs with a signficant BLAST hit. Subset of contigs with a significant BLAST hit and at least one SNP. In other words, the SNPs described in this table are a subset of the 877 we identified, but include all of those associated with a particular gene.
Format: DOC Size: 108KB Download file
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