A) Frequency of miRNAs sequenced. The number of reads of each miRNA is shown. The contribution of each miRNA to the total number of reads was variable and a small number of miRNAs generated approximately half of the total number of reads. B) Size distribution of sequenced miRNAs. The length of reads matching the ZF genome ranged from 15 to 25 nucleotides. Most miRNA reads had between 19–22 nt in length, thus confirming the high quality of the small RNA libraries used in the pyrosequencing run.
Soares et al. BMC Genomics 2009 10:195 doi:10.1186/1471-2164-10-195