Correlation between mRNA expression levels measured by Affymetrix microarrays and RNA-Seq. mRNA expression levels measured by RNA-Seq in two pooled samples of 5 individuals and by microarrays in the same samples (A) or in 5 independent individual samples (B). Shown are expression levels of 8,441 and 4,758 genes, respectively, expressed above background on at least one of the microarrays in a given experiment and represented by at least two independent sequence reads in RNA-Seq (see Methods for details). (C and D) Person correlation coefficients (r) from comparisons between RNA-Seq and microarray measurements based on each microarray separately and on average expression from all possible microarray combinations for two pooled and 5 individual samples, respectively (see Additional file 1: Table S3 for details).
Fu et al. BMC Genomics 2009 10:161 doi:10.1186/1471-2164-10-161