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Open Access Research article

Gene expression signature of cerebellar hypoplasia in a mouse model of Down syndrome during postnatal development

Julien Laffaire1,8, Isabelle Rivals2, Luce Dauphinot1,8, Fabien Pasteau1, Rosine Wehrle3,4, Benoit Larrat5, Tania Vitalis1, Randal X Moldrich1,6, Jean Rossier1, Ralph Sinkus5, Yann Herault7, Isabelle Dusart3,4 and Marie-Claude Potier1,8*

Author Affiliations

1 Laboratoire de Neurobiologie, CNRS UMR7637, ESPCI, Paris, France

2 Equipe de Statistique Appliquee – ESPCI, Paris, France

3 Neurobiologie des Processus Adaptatifs, CNRS UMR7102, Paris, France

4 UPMC, Paris, France

5 Laboratoire Ondes et Accoustique, UMR7587, ESPCI, Paris, France

6 The Queensland Brain Institute, St Lucia, Australia

7 IEM, CNRS UMR6218, Orleans, France

8 CRICM, CNRS UMR7225, INSERM UMR975, UPMC, CHU Pitie-Salpetriere, Paris, France

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BMC Genomics 2009, 10:138 doi:10.1186/1471-2164-10-138

Published: 30 March 2009

Additional files

Additional File 1:

Using magnetic resonance imaging we measured the volumes of whole brain and cerebellum of six male adult Ts1Cje and nine euploid littermates with a very high-resolution of 100 μm. The obtained segmentation (C) using implemented snake evolution algorithm was manually corrected in the three orthogonal planes: horizontal (A), sagital (B) and coronal (D). Cb: cerebellum.

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Additional File 2:

Fifty six samples from individual cerebella at P0, P3, P7 and P10 were hybridized on 28 two-color microarrays. On each microarray we hybridized a Ts1Cje sample versus an euploid sample. In addition on the same microarray we compared samples from mice of the same age or with a maximum difference of 4 days (P0 versus P3, P3 versus P7 or P7 versus P10). Each sample is identified by a letter and a number referring to the litter and to the pup in the litter respectively. Trisomic and euploid samples at each stage of development were equally labelled by Cy3 (in green) and Cy5 (in red).

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Additional File 3:

For each gene, the expression ratio, the p-value of the ANOVA (α = 5%) or the Student t-test (α = 5%) and the gene ontology are given. In bold are three-copy genes and in grey are genes that passed the False Discovery Rate controlling procedure of Benjamini and Hochberg (q < 0.40).

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Additional File 4:

For each gene, we indicate the Ts1Cje/euploid ratio at P0, P3, P7 and P10 and the expression ratio between P0–P3, P0–P7 and P0–P7 in euploid mice. In grey, non significant results (p > 0.05). NE: non expressed.

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Additional File 5:

In green are genes significantly downregulated and in red genes significantly overexpressed (Student t-test, α = 5%). NE: non expressed. Genes are ranked according to their chromosomal location (top to bottom: centromeric to telomeric).

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Additional File 6:

UPL refers to the number of the probe in the Roche Universal Probe Library.

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