Table 6

Primer/probe list for UT-quantitative PCR and UT-digital PCR

Primers for Standard 454 libraries:

Forward:

5'-CCATCTCATCCCTGCGTGTC-3'

Reverse:

5'-CCTATCCCCTGTGTGCCTTG-3'

UTBP-1:

5'-GGCGGCGACCATCTCATCCCTGCGTGTC-3'

Primers for 454 MID/Paired end libraries:

Forward:

5'-GCCTCCCTCGCGCCATCAG-3'

Reverse:

5'-GCCTTGCCAGCCCGCTCAG-3'

UTBP-2:

5'-GGCGGCGAGCCTCCCTCGCGCCATCAG-3'

Primers for Solexa libraries:

Forward:

5'-ACACTCTTTCCCTACACGA-3'

Reverse:

5'-CAAGCAGAAGACGGCATA-3'

UTBP-3:

5'-GGCGGCGAACACTCTTTCCCTACACGA-3'

Universal probe sequence:

UPL#149

5'-CCGCCGCT-3'


White et al. BMC Genomics 2009 10:116   doi:10.1186/1471-2164-10-116

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