Table 5

Thermocycling parameters for UT-quantitative PCR and UT-digital PCR

Standard Adapters 454

UT-dPCR & UT-qPCR

MIDs/Paired-end

UT-qPCR

MIDs/Paired-end

UT-dPCR

Solexa

UT-dPCR


Hot Start

95C, 3 mins

95C, 3 mins

95C, 3 mins

95C, 10 mins

Denaturation

94C, 30 secs

95C, 3 secs

95C, 15 secs

95C, 15 secs

Annealing

60C, 30 secs

65C, 30 secs

65C, 30 secs

60C, 1 min

Extension

72C, 45 secs

-

-

-

Cycle

40

40

40

40


White et al. BMC Genomics 2009 10:116   doi:10.1186/1471-2164-10-116

Open Data