Table 4

Solexa trace library generation & sequence results

Solexa Libraries

Input (ng)a

DNA Library (total molecules by UT-dPCR)*/ul

Average number of clusters generated per tile

Total number of reads

% mapping to Human reference (hg 18)


Plasma DNA Sample 1

3.2

1.07 × 1011

115998

11599833

51.5%

Plasma DNA Sample 2

3.6

7.88 × 1010

114548

11454876

52.7%

Plasma DNA Sample 3

2.7

7.17 × 1010

118516

11851612

56.1%

Plasma DNA Sample 4

2.6

6.03 × 1010

150414

15041417

49.7%

Plasma DNA Sample 5

5.6

7.17 × 1010

119104

11910483

56.1%

Plasma DNA Sample 6

2.4

7.23 × 1010

120974

12097478

55.4%

Whole Blood DNA Sample

2.1

6.30 × 1010

151201

15120171

50.5%


a Quantified by digital PCR with human specific primers on a unique locus taking 6.6 pg per cell.

White et al. BMC Genomics 2009 10:116   doi:10.1186/1471-2164-10-116

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